Profile

Xu’ai Lin, Ph.D.
Department of Medical Microbiology & Parasitology
School of Medicine, Zhejiang University, Hangzhou
866 Yuhangtang Road, Xihu area
Phone: 86-571-88208294 (lab); 13958069102 (cell)
Lxai122@163.com

Education
Ph.D. 2006, Biological Sciences, the Chinese Agricultural Academy of Sciences, China
Promoter analysis of ubiquitin and polyhedron genes, and the utilization of baculovirus expression system.
Advisor: Dr. Zhifang Zhang and Prof. Guifang Shen

M.S. 2003, Biological Sciences, Shandong Agricultural University and the Chinese Agricultural Academy of Sciences, China
Cloning and expression of cysteine protease from BmNPV using Bombyx mori bioreactor.
Advisor: Prof. Weiguo Li and Dr. Zhifang Zhang

B.S. 2000, Economic Animal Production, Shandong Agricultural University, China
Advisor: Prof. Zhimei Mu

Postdoctoral Training and Employment
09/2006-09/2008, School of Medicine, Zhejiang University
Development of a novel leptospire vaccine based on the epitopes of outer membranes from Leptospira interrogans
Advisor: Prof. Jie Yan

10/2008-present, School of Medicine, Zhejiang University
Dissecting the uncharacterized, novel virulence mechanisms that are involved in infection of some important bacteria and development of a novel leptospire vaccine
1) Role of c-di-GMP in the regulation of bacterial infection and drug resistance.
2) Epigenetic mechanism of the survival of leptospires in host cells.
3) Development of a MAP vaccine based on the epitope from outer membrane proteins of leptospire.

Research grants
Responsible person:
1. China National Natural Science Foundation “Molecular mechanism of c-di-GMP on the regulation of leptospire virulence and immune escape” (No. 81271781, 2013.01-2016.12)
2. Zhejiang Province Natural Science Foundation “Mechanism of c-di-GMP on the virulence of leptospira interrogans” (No. LY12H19007, 2012.01-2014.12)
3. Postdoctoral foundation “Epitope analysis of outer membrane proteins from Leptospira and expression of multi-epitope protein” (No. 20060401069, 2006.09-2008.09)
4. China National Natural Science Foundation “Role of hemolysis on the pathogenesis of leptospira interrogans and related signal pathway” (No. 30700034, 2008.01-2010.12)
Participant:
5. China National Natural Science Foundation of cross-strait cooperation projects “Pathogenesis mechanism of leptospira interrogans and interaction of the pathogen and host” (No. 81261160321, 2013.01-2015.12)
6. China National Natural Science Foundation “Pathogenesis role of leptospira interrogans on different host and cells and mechanism on the transmission and release of pathogen” (No. 81171534, 2012.01-2015.12)

Selected publications
1. Wang H, Wu Y, Ojcius DM, Yang XF, Zhang C, Ding S, Lin X (corresponding author), Yan J (corresponding author). Leptospiral Hemolysins Induce Proinflammatory Cytokines through Toll-Like Receptor 2-and 4-Mediated JNK and NF-κB Signaling Pathways. PLoS One. 2012 Aug, 7(8): e42266.
2. Lin X, Sun A, Ruan P, Zhang Z, Yan J. Characterization of Conserved T- and B-Cell Combined Epitopes in Leptospira interrogans Major Outer Membrane Protein OmpL1 and LipL41. BMC Microbiol. 2011 Jan, 11, 21.
3. Lin X, Zhao J, Qian J, Mao Y, Pan J, Li L, Peng H, Luo Y, Yan J. Identification of immunodominant B- and T-cell combined epitopes in outer membrane lipoprotein LipL32 and LipL21 of Leptospira interrogans. Clin Vaccine Immunol. 2010 May, 17(5): 778-783.
4. Lin X, Chen Y, Yi Y, Zhang Z. Baculovirus immediately early 1, a mediator for homologous regions enhancer function in trans. Virol J. 2010 Feb, 7(1): 32.
5. Lin X, Chen Y, Lu Y, Yan J, Yan J. Application of loop-mediated isothermal amplification method for the detection of pathogenic leptospira. Diagn Micr Infec Dis. 2009 Mar, 63: 237-242.
6. Lin X, Chen Y, Yan J. Recombinant multiepitope protein for diagnosis of leptospirosis. Clin Vaccine Immunol. 2008 Nov, 15(11): 1711-1714.
7. Lin X, Chen Y, Yi Y, Yan J, Zhang Z. Promoter analysis of Bombyx mori nucleopolyhedrovirus ubiquitin gene. J Microbiol. 2008 Aug, 46(4): 429-435.
8. Lin XA, Chen Y, Xu WH, Yi YZ, Zhang ZF. Characterization of Autographa californica nucleopolyhedrovirus ubiquitin gene promoter. Z Naturforsch [C]. 2008 Mar-Apr, 63c: 277-283.
9. Lin X, Zhang W, Chen Y, Yao B, Zhang ZF. Overexpression of celB Gene Coding for β-Glucosidase from Pyrococcus furiosus Using Baculovirus Expression Vector System in Silkworm, Bombyx mori. Z Naturforsch [C]. 2006 Jul-Aug, 61(7-8): 595-600.
10. Zhang L, Zhang CL (co-first author), Ojcius M., Zhao JF, Lin XA, Mao YF, Li LW, Yan J. The mammalian cell entry (Mce) protein of pathogenic Leptospira species is responsible for RGD motif-dependent infection of cells and animals. Mol Microbiol. 2012 Feb, 83(5): 1006-1023.
11. S Quan, J Wang, X Xia, X Lin, J Yan. Protective effect of puerarin form the roots of Pueraria lobata against systemic inflammatory response syndrome by regulating the levels of related cytokines. J Med Plants Res. 2011 July, 5(13): 2917-2921.
12. Chen Y, Lin X, Yi Y, Lu Y, Zhang Z. Construction and application of a baculovirus genomic library. Z Naturforsch [C]. 2009 Jul-Aug, 6 64 c, 574-580 .
13. Luo D, Xue F, Ojcius DM, Zhao J, Mao Y, Li L, Lin X, Yan J. Protein typing of major outer membrane lipoproteins from Chinese pathogenic Leptospira spp. and characterization of their immunogenicity. Vaccine. 2009, 28(1): 243-255.
14. Chen Y, Zhu Z, Lin X, Yi Y, Zhang Z. Overexpression and Characterization of appA Phytase Expressed by Recombinant Baculovirus Infected Silkworm. J Microbiol. Biotechnol. 2005 Jan,15(3): 466-471.
15. Chen Y, Yao B, Zhu Z, Yi Y, Lin X, Zhang Z, Shen G. A constitutive super-enhancer: homologous region 3 of Bombyx mori nucleopolyhedrovirus. Biochem Biophys Res Commun. 2004 Jun, 318(4): 1039-1044.

Patents:
1. One method for the preparation of thermostabe lactose (ZL200610001785.X)
2. One method for the purification of small DNA from agarose gel (ZL20071007183.6)
3. One method for the detection of leptospire (ZL200710164460.8)

Honors
Seeking young scholars of Zhejiang University