Profile

Academic community:2016 – Zhejiang Association of MicrobiologyEducation and research experience：1997 - 2001 BS (Chemistry, Peking University, China)2001 - 2007 PhD (Biochemistry, University of Pennsylvania, USA) 2007 - 2008 Postdoctoral research, University of Pennsylvania2008 - 2015 Postdoctoral research, Harvard Medical School2015 - present Professor of Pathogen Biology, Zhejiang University School of MedicineResearch direction: 1. Molecular mechanisms of viral pathogenesis and latency.2. Virus-host interactions3. Therapeutics for treating latent viral infectionResearch features: Our research is mainly focused on herpes simplex virus, which causes diseases including cold sores, keratitis, genital herpes and encephalitis. It can also undergo latency, which allows the virus to persist in the host. There is currently no cure for HSV disease. We are carrying out the following projects to study viral pathogenesis and latency and to explore new therapeutics: 1) Investigation of the impact of viral and host miRNAs on viral latency, as well as the related molecular mechanisms. 2）The mechanism that a viral protein antagonizes host antiviral immunity. 3) The use of genome editing tools in the study of viral genetics and in the treatment of viral latent infection. The technologies we used include next-generation sequencing, various cell and mouse models, CRISPR/Cas9, in addition to basic molecular biological techniques. Finally by collaborating with other laboratories, we also started working with other viruses.Publications1) Raja P, Lee JS, Pan D, Pesola J, Coen DM, Knipe DM. A herpesviral lytic protein regulates the structure of latent viral chromatin. mBio 7, e00633-16 (2016)2) Pan D, Flores O, Umbach JL, Pesola J, Peris B, Cullen BR, Coen DM. A neuron-specific host microRNA targets herpes simplex virus-1 ICP0 expression and promotes latency. Cell Host Microbe 15, 446-456 (2014)3) Pan D, Hart IJ, Hopkins M, Kirwan R, Kaye S, Coen DM. Common and new acyclovir resistant herpes simplex virus-1 mutants causing bilateral recurrent herpetic keratitis in an immunocompetent patient. J. Infect. Dis. 209, 345-349 (2014).4) Pan D, Coen DM. Net -1 frameshifting on a noncanonical sequence in a herpes simplex virus drug-resistant mutant is stimulated by nonstop mRNA. Proc. Natl. Acad. Sci. U.S.A. 109, 14852-14857 (2012).5) Pan D, Coen DM. Quantification and analysis of thymidine kinase expression from acyclovir-resistant G-string insertion and deletion mutants in herpes simplex virus-infected cells. J. Virol. 86, 4518-4526 (2012).6) Liu H, Pan D, Nierhaus K., Kitikawa M, Cooperman BS. Interrupted catalysis: The EF4 (LepA) effect on reverse translocation. J. Mol. Biol. 396, 1043-1052 (2010).7) Pan D, Qin H, Cooperman BS. Synthesis and functional activity of tRNAs labeled with fluorescent hydrazides in the D-Loop. RNA 15, 346-354 (2009).8) Pan D, Zhang CM, Kirillov S, Hou, YM, Cooperman BS. Perturbation of the tRNA tertiary core differentially affects specific steps of the elongation cycle. J. Biol. Chem. 283, 18431-18440 (2008).9) Walker SE, Shoji S, Pan D, Cooperman BS, Fredrick K. Role of hybrid tRNA-binding states in ribosomal translocation. Proc. Natl. Acad. Sci. U.S.A. 105, 9192-9197(2008).10) Zhang CM, Liu C, Christian T, Gamper H., Rozenski J, Pan D, Randolph JB, Wickstrom E, Cooperman BS, Hou YM. Pyrrolo-C as a molecular probe for monitoring conformations of the tRNA 3’ end. RNA 14, 2245-2253 (2008).11) Mandecki W, Bharill S, Borejdo J, Cabral D, Cooperman BS, Farrell I, Fetter L, Goldman E, Gryczynski Z, Jakubowski H, Liu H, Luchowski R, Matveeva E, Pan D, Qin H, Tennant D, Gryczynski I. Fluorescence enhancement on silver nanostructures: studies of components of ribosomal translation in vitro. Proc. SPIE 6862, 68620T (2008).12) Pan D, Kirillov S, Cooperman BS Kinetically competent intermediates in the translocation step of protein synthesis. Mol. Cell 25, 519-529 (2007).13) Grigoriadou C, Marzi S, Pan D, Kirillov S, Gualerzi CO, Cooperman BS. The translation fidelity function of IF3 during transition from the 30S initiation complex to the 70S initiation complex. J. Mol. Biol. 373, 551-561(2007).14) Wang Y, Qin H, Kudaravalli RD, Kirillov SV, Dempsey GT, Pan D, Cooperman BS, Goldman YE, Single molecule structural dynamics of EF-G.Ribosome interaction during translocation. Biochemistry 46, 10767-10775 (2007).15) Pan D, Kirillov S, Zhang CM, Hou YM, Cooperman BS. Rapid ribosomal translocation depends on the conserved 18-55 base pair in P-site transfer RNA. Nat. Struct. Mol. Biol. 13, 354-359 (2006). 16) Seo HS, Kiel M, Pan D, Raj VS, Kaji A, Cooperman BS. Kinetics and thermodynamics of RRF, EF-G, and thiostrepton interaction on the Escherichia coli ribosome. Biochemistry 43,12728-12740 (2004).