Profile

PERSONAL DETAILS

Name: Jie Sun
Sex: Female
Age: 35
Title: Ph. D., Associate professor, Attending Physician of Hematology
Office: Bone Marrow Transplantation Centre, Department of Hematology, The First Affiliate Hospital, Medical College of Zhejiang University.



EDUCATION AND ACADEMIC QUALIFICATIONS

Education

Sep. 1999 to Jul. 2004
Ph.D., Medical College, Zhejiang University

Sep. 1991 to Jul. 1996
Bachelor of Medicine, Zhejiang Medical College

Employment History

Aug. 2004 to present
Associate Professor, Attending Physician, Bone Marrow Transplantation Centre, Hematology Department, The First Affiliated Hospital, Medical College of Zhejiang University

Aug. 1996 to May. 2003
Resident Physician, Hematology Department, Affiliated Hospital of Zhejiang Traditional Medicine University

RESEARCH EXPERIENCE

1996 to 1999: During my master’s course, I engaged in the research of my advisor Prof. Huang’s project (a National Natural Science Foundation of China 39870339):” Study on the mutation and expression of hTRF1 in acute leukemia cells”. Under the directions of Prof. Chen Saijuan of Shanghai Institute of Hematology, RUIJIN Hospital, I completed the mutation detection of whole 10 exons of hTRF1 in normal and acute leukemia samples and found several genome variants. I also investigated the expressions of hTRF1, together with some other telomere binding proteins such as TRF2, TANK1, TANK2 and pinx1, in leukemia cell lines cells, leukemia samples and gastric cancer samples, and obtained some new findings, papers were published on J Zhejiang Univ Sci B and Blood as abstracts.
1999 to 2003: With the findings, I assisted with my advisor prof. Huang for the
application of a new project named “Study the network of cell cycle
regulation” and obtained the financial assistance by
The National Basic Research Program of China (973 program
2002CB713700). The aim of our study is to clarify the mechanism of
telomere binding proteins in their regulation with cell proliferation and
differentiation and to find new regulators. I am the main member of
the research group. Under the directions of Prof. Yao Xuebiao of
University of Science and Technology of China in his LCD Lab, I studied the expression of telomeric proteins and their probable regulation of telomerase during the differentiation of all-trans-retinoic
acid–responsive and –resistant acute promyelocytic leukemia cells,
the paper is published on Int J Hematol.
2003 to present: Collaborated with my colleagues, I studied on the regulation of
telomere binding proteins on human mesenchymal stem
cells(hMSCs) and found PML may play some special roles on the
proliferation or differentiation of hMSCs. Based on these findings, I
applied a project of National Natural Science Foundation of China
named” Study on the mechanism of PML on the regulation of
proliferation and differentiation of hMSCs” (30600256). Now, I am
still working on this project.

SKILLS

1. Electrophoresis - SDS-PAGE, native, agarose, two-dimensional
routine PCR and real-time PCR
2. Molecular biology - plasmid preparation, restriction digest analysis, DNA sequencing, in-vitro transcription, transformation.
3.Co-immunoprecipitation
4. Basic cell culture; media preparation;culturing techniques; culture maintenance. Mesenchymal stem cell culture. MTT and NBT tests.
5. Mutation detection, DNA extraction, sequencing result analysis
6. Protein isolation/purification; Western blots, immunofluorescence, ELISA
7. cell separation by immunomagnetic beads
Research Grants Awarded

1. Study on the mechanism of PML on the regulation of proliferation and differentiation of hMSCs. National Natural Science Foundation of China (30600256). 1997-2009. RMB 210,000.
2. Study Matrine on its releasing ATRA resistance in acute promyelocytic leukemia cell line cells. Zhejiang Province Traditional Medicine Program (2008G1010). 2008-2010 RMB 20,000
3. Freeze-dry as a new method for hMSCs preservation. Young Scholar Project of Zhejiang University. 2006 RMB 10,000
4. Study on the function of a new TRF1 binding factor TRF1BP1 and its regulation on the cell cycle. Jianping Lan et al. National Natural Science Foundation of China (30570941). 2006 RMB 80,000
5. Study the regulation of TANK1 on the expression of hTERT. The project of education department, Zhejiang Province (20061017). RMB 20,000

PRESENTATIONS AND PROFESSIONAL ACTIVITIES

1. Oral Presentation: /"Study on the expression and mutation of Human Telomeric Repeat Binding Factor (hTRF-1) in 10 malignant hematopoiatic cell lines./" Seventh International Human Genome Meeting, HGM2002.
2. Oral Presentation: “The Regulation of Telomere Binding Proteins on Telomerase during Differentiation of ATRA Responsive and Resistant NB4 Cells.” The 10th International Conference on Differentiation Therapy.

AWARDS

1. 2003 GuangHua Scholarship of Zhejiang University.
2. 2003 Silver Award of Zhejiang Government on scientific and technological progress.

PUBLICATIONS
1. Sun J, Huang H. Telomere binding proteins-a new study field. GuoWaiYiXue ShuXueJiXueYeXueFenCe. 2001, 24（5）：402-405.
2. Sun J，Huang H，Yu RX.Treat myelodysplastic syndrome with stem cell transplantation. GuoWaiYiXue ShuXueJiXueYeXueFenCe. 2002, 25（1）:57-60
3. Sun J , Huang He，Song Huaidong, Wu Xinyan. Pseudogenes of Human Telomeric Repeat Binding Factor (hTRF1) and related Transchromosomal Duplications。Chinese Medical Journal Web prints 2002, 115(6):10
4. Sun J, Huang H, Song HD. Study on the gemome structure of telomeric repeat binding factor1 and its pseudogenes. Journal of Zhejiang University(Medical Science). 2003, 32（5）:407-411.
5. Sun J, Huang H, Zhu YY. Study on the Expression of Tankyrase in Malignant Hematopoietic Cells and Its Relation with Telomerase Activity。Journal of Experimental Hematology. 2004, 12（1）:11-15.
6. Sun J, Huang H, Zhu YY. Study on the mutation of Human Telomeric Repeat Binding Factor 1 gene in malignant hematopoietic cell lines. Chin J Hematol. 2004,（25）5: 269-272.
7. Jie Sun, Xiaoyu Lai, Yuanyuan Zhu, Lan JP, Li JY, Lai XY, Yu J. Expression of human telomere repeat binding factor1 (TRF1) in acute leukemia cells and its correlation with telomerase activities. Journal of Zhejiang University(Medical Science) 2004, 33(6):491-495.
8. Huang H, Sun J. The research progress of telomere binding proteins. Journal of Zhejiang University(Medical Science), 2004, 33（6）: 469-473.
9. Sun J, Huang H, Zhu Y, Jianping Lan, Jingyuan Li, Xiaoyu Lai, Jian Yu. The Expression of Telomeric Proteins and Their Probable Regulation of Telomerase during the Differentiation of All-trans-Retinoic Acid-Responsive and -Resistant Acute Promyelocytic Leukemia Cells. Int J Hematol. 2005 Oct;82(3):215-23. 2 Citations.
10. SUN J, Huang He, Zhu YY，Lan JP，Li JY，Lai XY，Yu J. Study on the expression and mutation of Human Telomeric Repeat Binding Factor (hTRF-1) in 10 malignant hematopoiatic cell lines. J Zhejiang Univ Sci B. 2005, 6(12):1141-1147.
11. Sun J, Huang H, Zhu YY. Lan JP, Yu J, Lai XY. Study on the expression of TRF1 and TANK1 in acute leukemia cells and there relation with telomerase activity. Chin J Hematol, 2006,27（1）：48-50.
12. Sun J, Tan YM, Huang H Lan JP, Lai XY. Study on expression of telomerase inhibitor Pinx1 in leukemia cells and its correlation with telomerase activity. Chinese Journal of Pathophysiology, 2006, 21（9）:1725-1728.
13. Li R, Sun J. New insight on the vitro store of human mesenchymal stem cells. International Journal of Transplantation and Hemopurification.2008, 6（2）: 29-32.
14. Wang Z, Sun J. Progression on freeze-drying preservation technology to human nucleolate cells. International Journal of Transfusion and Hematology 2008, 3（31）:271-274.
Abstracts:
1. Huang H，Sun J. Characterization of the Human Telomeric Repeat Binding Factor (hTRF1) Gene and Its Four Pseudogenes. Blood 2001.106b.4316.
2. Huang H, Sun J. A study of hTRF1 gene mutation in malignant hematopoietic cell lines cells. Blood 2002. 100(11)(part 2): 207b, 4336.
3. Huang H; Sun J; Zhu, YY. Study on the expression of tankyrase in malignant hematopoietic cells and its relation with telomerase activity. Blood 2003, 102 (11 Part 2): 190B
4. Huang H, Sun J, Zhu YY, Lan JP, Lai XY. The Regulation of Telomere Binding Proteins on Telomerase during Differentiation of ATRA Responsive and Resistant NB4 Cells. Blood, 2004, 104 (11 Part 1): 925A: 3390.
5. Sun J, Huang H, Zhu YY, Lan JP, LAI XY. Study on expression of telomerase inhibitor Pinx1 in leukemia cells and its correlation with telomerase activity. Blood, 2005, 106(11): 4375.