Profile

Academic community and social service:
• The member of Chinese Society of Immunology.
• The teaching secretary of Department of Immunology, Medical College of Zhejiang University (2007.4-2009.10)
• The class Adviser of Clinical Medicine of Seven-year-program, Medical College of Zhejiang University (2007.1-2009.10)

Education and research experience：
• B.S. (1992.9-1995.6) Clinical Medicine, Zhejiang Medical University
• M.S. (1998.9-2001.6) In Immunology, Medical College of Zhejiang University
• Ph.D. (2001.9-2004.6) In Oncology/Immunology, Medical College of Zhejiang University
• Lecturer (2004.8-2007.12) The department of Immunology, Medical College of Zhejiang University
• Associated Professor (2007.12-present) The department of Immunology, Medical College of Zhejiang University
• Postdoctoral Associate (2009.10-present) The department of Immunology, Duke university, USA

Research directions
1. Molecular immunology: We reported a novel apoptosis-inducing protein, LAPF, which derived from human bone marrow stromal cells. LAPF specifically interacted with phosphorylated p53 (Ser15) and recruited phosphorylated p53 (Ser15) to lysosomes, resulting in caspase-independent apoptosis via a lysosomal-mitochondria pathway. These data were published in J Biol. Chem. and Cancer Res.

2. Cellular immunology: My present research work is focus on the role of autophagy in CTL cytotoxicity in Duke University. Autophagy is important process to maintain cell survival or induce apoptosis under different conditions. Interestingly, we found the CTL cytotoxicity increased in autophagy deficient mouse model. The mechanism need to be elucidated. On the other hand, we demonstrated the preventive effect of mIL-12 gene on allergic asthma. Intramuscularly delivery of mIL-12 plasmid might reduce airway hyperreactivity through the decreased expression of CD44 and CD49d on leukocytes in BALF and pulmonary infiltration of leukocytes. These data were published in Inflamm Res.

Grants
1. National Natural Science Foundation, China (as PI): “The role and mechanism of a novel apoptosis-inducing protein LAPF in the apoptosis of monocyte/macrophage” (30500446).

2. Natural Science Foundation, Zhejiang province, China (as PI): “The therapeutic effects and mechanism of apoptosis-inducing protein LAPF in human breast cancer” (Y206176).

3. Department of Education Grant, Zhejiang Province (as PI): “The functional study of a novel apoptosis-inducing protein LAPF” (20061698).

4. National Natural Science Foundation, China (as co-PI): “The negative effects of PECAM-1 on TLR4 signal transduction in macrophage” (30671909).
Publications
7. Chen W, Li N, Chen T, Han Y, Li C, Wang Y, He W, Zhang L, Wan T, Cao X. The lysosome-associated apoptosis-inducing protein containing the pleckstrin homology (PH) and FYVE domains (LAPF), representative of a novel family of PH and FYVE domain-containing proteins, induces caspase-independent apoptosis via the lysosomal-mitochondrial pathway. J Biol Chem. 2005, 280(49):40985-40995. (Impact factor: 5.85)

8. Li N, Zheng Y, Chen W, Wang C, Liu X, He W, Xu H, Cao X. Adaptor protein LAPF recruits phosphorylated p53 to lysosomes and triggers lysosomal destabilization in apoptosis. Cancer Res. 2007 Dec 1;67(23):11176-11185. (As co-first author, Impact factor: 7.656)

9. Li H, Xie Q, Wang H, Shao C, Chen W. Intramuscular delivery of mIL-12 gene reduces the expression of CD44/CD49d on pulmonary leucocytes and inhibits ovalbumin-induced airway hyperreactivity. Inflamm Res. 2008 Jan;57(1):11-17. (As corresponding author, Impact factor: 1.485)

10. Chen T, Guo J, Yang M, Han C, Zhang M, Chen W, Liu Q, Wang J, Cao X. Cyclosporin A impairs dendritic cell migration by regulating chemokine receptor expression and inhibiting cyclooxygenase-2 expression. Blood. 2004, 103(2):413-421. (Impact factor: 10.13)

11. Li C, Liu Q, Li N, Chen W, Wang L, Wang Y, Yu Y, Cao X. EAPF/Phafin-2, a novel endoplasmic reticulum-associated protein, facilitates TNF-alpha-triggered cellular apoptosis through endoplasmic reticulum-mitochondrial apoptotic pathway. J Mol Med. 2008 Apr;86(4):471-484. (Impact factor: 5.157)

12. He W, Liu Q, Wang L, Chen W, Li N, Cao X. TLR4 signaling promotes immune escape of human lung cancer cells by inducing immunosuppressive cytokines and apoptosis resistance. Mol Immunol. 2007 Apr;44(11):2850-2859. (Impact factor: 4.768)

Edit the monograph and teaching material
“Medical immunology”, chief editor: Jianping Pan, Zhejiang University Press, August 2006.