Major: Optical & Electrical Engineering

     Department: Department of Optical Engineering, College of Info Science and
                            Engineering

     Email: caesarhx@gmail.com

Mentor: Dr. Yong Chen

Department: Mechanical & Aerospace Engineering

Title of research project: Setting-up & Improvement of FCS System and its further application

Xiang Hao is a fourth year undergraduate Optical Engineering Major. Now he is doing research on FCS System. Fluorescence correlation spectroscopy (FCS) was first introduced by Madge, Elson and Webb in 1972, as one of the many different modes of high-resolution spatial and temporal analysis of extremely low concentrated biomolecules. In contrast to other fluorescence techniques, the parameter of primary interest is not the emission intensity itself, but rather spontaneous intensity fluctuations caused by the minute deviations of the small system from thermal equilibrium. All of them are very important and essential in the biochemical research, biophysics and chemistry.

Now the FCS Systems are widely used in bio-analysis., but its system is not so satisfied in many places. The purpose of the first step of this project is trying to redesigning and setting up a new FCS System and let it fit the requirement of the experiment, but with less weight and volume, spending less money, and easy to take apart and re-set up if it doesn’t work in the future. Based on these, our group will use it to do some further research which belongs to the second step. Xiang Hao’ s work now is just focusing on the first step. The work of him both contain designing of the software, and the hardware. For the software, it should do all the mathematics analysis; and the hardware, its function is detect the intensity of the fluorescence. Mr. Xiang Hao plan to finish the code of the software in 4 weeks, now the work has been completed. Three parts of the hardware(the optical table, power supply and detector module) also have been finished by Sep. 12. When he come back to Zhejiang University, he will still keep in touch with this project and try to change the Process-Oriented to Object-Oriented one.

Xiang Hao love the environment of lab in UCLA and he regard here as a brilliant place for his further research life. He always said:” I come, I see, so I should conquer something here.”

 　　在机械与航天工程学系Yong Chen教授的研究小组中进行实验工作。所在实验室主要从事各种材料（生物和硅材料为主）及相关纳米器件的制作工艺和特性分析。郝翔所从事的工作是完成一台荧光自相关分析仪（简称FCS）的设计、安装、改进和调试工作。由于面对的是一个完整的系统，因此整个工作分为软件设计和硬件安装两个部分。具体包括：使用Matlab编辑数学算法、建立数学模型进行分析；安装和调试样品信号探测系统；使硬件各模块间、硬件和软件间的工作紧密匹配。经过两个月的努力，目前整个软件的核心算法部分、硬件中的平衡模块、电源模块和探测模块已经完成设计安装并测试通过。新完成的FCS系统将被用于进行生物细胞内反应进行速率及分子扩散速度的检测，具有广泛的应用领域和应用价值。